Do I need at least 2 RAM slots full for proper functionality?

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If you are in a dilemma about which slots to put RAM in, this 1 minute of discussion can make

In this video, we have elaborately discussed which ram slots 1 3 vs 2 4 and different channel But with 32GB Vengeance RGB PRO SL ram Same slot does not show the ram stick, the second slot from the right Seems like it's a software

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